Optimization of enzymatic hydrolysis of visceral waste proteins of beluga sturgeon Huso huso using Alcalase
Mahmoudreza Ovissipour1, Majid Taghiof2, Ali Motamedzadegan3, Barbara Rasco4, Abbas Esmaeili Molla5
1Department of Fisheries, Gorgan Agricultural Sciences and Natural Resources University, Iran; Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway 2Department of Chemistry, Islamic Azad University of Tonekabon, Tonekabon, Iran 3Department of Food Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran 4Department of Food Science and Human Nutrition, Washington State University, Washington,USA 5Shahid Rajaee Sturgeon Fish Farm, Sari, Iran.
Fish protein hydrolysate (FPH) was produced from the viscera of beluga sturgeon (Huso huso). Using response surface methodology (RSM) and a factorial design to minimize enzyme use and to model the degree of hydrolysis (r2 = 0.94), the hydrolysis conditions (temperature, time and enzyme activity) were optimized. The optimum conditions were: 50°C, 120 min, protease (Alcalase® 2.4 L) activity on 34 AU/kg protein. The hydrolysates of beluga visceral protein have a relatively high protein (66.43%) and low lipid (1.34%) content. The chemical score of the hydrolysate indicated that the amino acid profile of beluga sturgeon hydrolysate fulfills human adult nutritional requirements, with phenylalanine being the first limiting and predominant amino acid in the hydrolysate.
Keywords: Fish protein hydrolysate, Sturgeon visceral protein, Alcalase, Process optimization