Effects of temperature, salinity, illumination and Cu2+ on oxygen consumption of juvenile Chinese sturgeon Acipenser sinensis
Jianyi Liu1, Ming Duan1,2*, Liang Qu1,3, Longzhen Zhang1, Guangpeng Feng1, Tao Zhang1, Junli Hou1, Wengang Yan1, Xiaorong Huang1, Ping Zhuang1,3*
1Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, 200090, China
2State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
3College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201316, China
The oxygen consumption rate (OCR) of juvenile Chinese sturgeon Acipenser sinensis (Body weight, range 5 - 32 g) were determined under different environmental conditions, including water temperature, salinity, illumination intensity and metal ion concentration [Cu2+] by single factor experimental analysis. OCR and opercula frequency (OF) increased with rising of water temperature. The linear relationships between water temperature (WT) and OF with OCR can be described as OCR = 0.018 WT + 0.016 (R2 = 0.988, P < 0.01), OCR = 2.737 OF + 77.726 (R2 = 0.64, P < 0.01), respectively. The acute exposure experiments showed that the OCR also increased initially with an increase in salinity. The linear relationships between salinity (S) and OCR can be described as OCR = 8.1 × 10-5 S3 – 3.01 × 10-3 S2 + 0.0263 S + 0.2729 (R2 = 0.995, P < 0.01). Three days of acclimation to different salinities, the OCRs decreased sharply relative to the first day exposure values. The equation OCR = - 6.29 × 10-4 S2 + 0.0238 S + 0.2797 (R2 = 0.988, P < 0.01) illustrates the linear relationship between salinity and OCRs after 3-day exposure. After 3-day acclimation, the OCRs increased also with increasing light intensity, except for the ambient light group (i.e., 2000 lx) and the group exposed to direct sunlight (i.e., 20000 lx). After 1-day exposure to different Cu2+ concentrations (C), the OCRs decreased rapidly in all of the groups. But the OCR level being gradually different with the increasing of Cu2+ concentration, OCR = 0.237e – 0.09 C (R2 = 0.898, P < 0.01). The results provided us with some useful guide lines which may apply into protection and cultivation management of this endangered species.
Keywords: Chinese sturgeon, Acipenser sinensis, Opercula frequency, Oxygen consumption rate, Temperature, Salinity, Illumination, Cu2+